![]() ![]() Capto Q ImpRes provided a recognition for guanylate bases when samples of deoxynucleotides or poly(dG) were examined. All deoxynucleotides and DNAs tested bound strongly to the chromatographic materials and could be eluted by a linear gradient of increasing NaCl concentration. These variations in biophysical properties have been utilized for comparative separations on these two resins. The intrinsic differences between single- and double-stranded DNAs are related to charge, hydrophobicity, size and three-dimensional structure. Capto Adhere carries a multimodal ligand which combines strong anion with aromatic recognition, while Capto Q ImpRes is a strong anion exchanger with a chemically similar ligand, but without a phenyl group. Burgess RR (2018) A brief practical review of size exclusion chromatography: rules of thumb, limitations, and troubleshooting.The differences in chromatographic behaviour of individual deoxynucleotides as well as small single-stranded and double-stranded DNA molecules have been examined for two resins from the Capto family: Capto Adhere and Capto Q ImpRes.Burgess RR (2018) A brief practical review of size exclusion chromatography: rules of thumb, limitations, and troubleshooting.Arakawa T, Ejima D, Tsumoto K, Obeyama N, Tanaka Y, Kita Y, Timasheff SN (2007) Suppression of protein interactions by arginine: a proposed mechanism of the arginine effects.GE Healthcare Life Sciences (2015) Capto™ MMC ImpRes.GE Healthcare Life Sciences (2013) Polishing of monoclonal antibodies using Capto™ MMC ImpRes in bind and elute mode. ![]() Chmielowski RA, Meissner S, Roush D, Linden TO, Glowacki E, Konietzko J, Nti-Gyabaah J (2014) Resolution of heterogeneous charged antibody aggregates via multimodal chromatography: a comparison to conventional approaches.Gao D, Wang LL, Lin DQ, Yao SJ (2013) Evaluating antibody monomer separation from associated aggregates using mixed-mode chromatography.Chen J, Tetrault J, Zhang Y, Wasserman A, Conley G, Dileo M, Haimes E, Nixon AE, Ley A (2010) The distinctive separation attributes of mixed-mode resins and their application in monoclonal antibody downstream purification process.Eriksson K, Ljunglöf A, Rodrigo G, Brekkan E (2009) MAb contaminant removal with a multimodal anion exchanger: a platform step to follow protein A.Gagnon P, Beam K (2009) Antibody aggregate removal by hydroxyapatite chromatography.Gagnon P, Ng P, Aberrin C, Zhen J, He J, Mekosh H, Cummings L, Richieri R, Zaidi S (2006) A ceramic hydroxyapatite based purification platform: simultaneous removal of leached protein A, aggregates, DNA, and endotoxins.McCue JT (2009) Theory and use of hydrophobic interaction chromatography in protein purification applications.Lu Y, Williamson B, Gillespie R (2009) Recent advancement in application of hydrophobic interaction chromatography for aggregate removal in industrial purification process.McCue JT, Engel P, Ng A, Macniven R, Thömmes J (2008) Modeling of protein monomer/aggregate purification and separation using hydrophobic interaction chromatography.Zhang X, Chen T, Li Y (2019) A parallel demonstration of different resins' antibody aggregate removing capability by a case study.He X (2015) Mixed-mode chromatography for mAb S aggregate removal: comparison of CHT™ ceramic hydroxyapatite, capto adhere, and capto adhere impres.Hall T, Wilson JJ, Brownlee TJ, Swartling JR, Langan SE, Lambooy PK (2015) Alkaline cation-exchange chromatography for the reduction of aggregate and a mis-formed disulfide variant in a bispecific antibody purification process.Vázquez-Rey M, Lang DA (2011) Aggregates in monoclonal antibody manufacturing processes.Bonnerjea J, Brake RP, Davis MR, Kellerman K, Preneta A (2008) Ion exchange chromatography and purification of antibodies, United States patent application publication US 2008/0312425 A1. ![]()
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